Methods of quantifying N2-(1-carboxyethyl)-2′-deoxy-guanosine (CEdG) and synthesis of oligonucleotides containing CEdG

US11179361B2 · kind B2 · utility

Assignee

• City of Hope · US

Inventors

• Samuel Rahbar · Calabasas, US
• Timothy W. Synold · Monrovia, US
• John Termini · Altadena, US
• Gerald WUENSCHELL · Duarte, US
• Daniel Tamae · Duarte, US

Key dates

• Filing date: Feb 2, 2018
• Grant date: Nov 23, 2021
• Expiry date: Feb 2, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/56
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Methods of quantifying a N2-(1-carboxyethyl)-2′-deoxyguanosine (CEdG) levels in biological samples and comparing those levels to known normal levels can diagnose a number of metabolic disorders or complications associated therewith, including diabetes, its associated complications, and cancer. Methods can also determine whether therapies for disorders are effective by measuring CEdG levels before and after treatment. Measurement of CEdG levels is achieved by using liquid chromatography electrospray ionization tandem mass spectrometry.