Patent · US Active

Method of preparing recombinant adeno-associated virus and recombinant baculovirus

US11180736B2 · kind B2 · utility

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1References

12Claims

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Assignee

WUHAN INSTITUTE OF PHYSICS AND MATHEMATICS, CHINESE ACADEMY OF SCIENCES · CN

Inventors

Yang-Chang Wu
Fuqiang XU · Shanghai, CN
Xiaobin He · Los Angeles, US

Key dates

Filing date	Jan 29, 2018
Grant date	Nov 23, 2021
Priority date	—
Expiry date	Jan 9, 2039

Classification

Technology area (CPC C)Chemistry; Metallurgy
CPC primaryC12N2800/22
WIPO fieldBiotechnology
WIPO sectorChemistry

Abstract

A method for producing a recombinant adeno-associated virus (rAAV) and a recombinant baculovirus virus, the method including: (1) infecting an insect host packaging cell line with a corresponding recombinant baculovirus integrated with an rAAV genome ITR-GOI (gene of interest flanked by AAV inverted terminal repeats) and an AAV Cap gene or AAV Rep gene; (2) culturing the host packaging cell line infected with the recombinant baculovirus, so as to produce the recombinant adeno-associated virus; and (3) separating and purifying the recombinant adeno-associated virus obtained in (2).

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.