HPPD variants and methods of use

US11180770B2 · kind B2 · utility

Assignee

• BASF Agricultural Solutions Seed US LLC · US

Inventors

• Marc Linka · Goldkronach, DE
• Fabien Poree · Frankfurt, DE
• Bernd Laber · Idstein, DE
• Gudrun Lange · Kelkheim, DE
• Christina Thies · Dormagen, DE
• Ernst Weber · Langenfeld, DE
• Michael Strerath
• Sandra Geske · Cologne, DE
• Heike Balven-Ross · Solingen, DE
• Nina Wobst · Wuppertal, DE
• Wayne Coco · Cologne, DE
• Nikolaus Pawlowski · Cologne, DE
• Jan Tebbe · Cologne, DE
• Manuel Dubald · Raleigh, US

Key dates

• Filing date: Mar 1, 2018
• Grant date: Nov 23, 2021
• Expiry date: Jul 5, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/52
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

In the present invention, HPPD polypeptides and plants containing them showing a full tolerance against one or more HPPD inhibitor herbicides belonging to various chemical classes are described. A set of mutant HPPD polypeptides have been designed which have either no or only a significantly reduced affinity to HPPD inhibitor herbicides and, at the same time, the rate of dissociation of the HPPD inhibitors of the mutant HPPD polypeptide is increased to such an extent that the HPPD inhibitors no longer act as slow-binding or slow, tight-binding inhibitors but, instead of this, have become fully reversible inhibitors. In particular, isolated polynucleotides encoding mutant HPPD polypeptides conferring tolerance to HPPD inhibitor herbicides belonging to various chemical classes are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.