Method for bioconversion of mogroside extracts into siamenoside I
US11180789B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Sep 26, 2019 |
| Grant date | Nov 23, 2021 |
| Priority date | — |
| Expiry date | Dec 10, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12P19/14
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for bioconversion of mogroside extracts into siamenoside I, comprising: using (1) DbExg1 protein or (2) a microorganism expressing the DbExg1 protein to contact or to cultivate with the mogroside extracts. The present invention can convert the mogroside extracts into siamenoside I, which has a higher sweetening power and better taste than other mogrosides. The method of the present invention uses a microorganism expressing the responsible enzyme, DbExg1, which was identified as a mediator of mogroside V conversion into siamenoside I in the present invention, so that siamenoside I was preferentially produced. Thus, the use of the method of the present invention provides a feasible approach to produce large quantities of the natural sweetener, siamenoside I, which can then be applied in several industries.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.