Patent · US Active

Use of Neuropilin-1 (NRP1) as a cell surface marker for isolating human cardiac ventricular progenitor cells

US11186820B2 · kind B2 · utility

3Cited by
15References
16Claims
0Family size

Assignee

Inventors

Key dates

Filing dateAug 22, 2018
Grant dateNov 30, 2021
Priority date
Expiry dateMar 15, 2039

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12N2533/90
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

The present invention provides NRP1 as a cell surface marker for isolating human cardiomyogenic ventricular progenitor cells (HVPs), in particular progenitor cells that preferentially differentiate into cardiac ventricular muscle cells. Additional HVP cell surface markers identified by single cell sequencing are also provided. The invention provides in vitro methods of the separation of NRP1+ ventricular progenitor cells, and the large scale expansion and propagation thereof. Large clonal populations of isolated NRP1+ ventricular progenitor cells are also provided. Methods of in vivo use of NRP1+ ventricular progenitor cells for cardiac repair or to improve cardiac function are also provided. Methods of using the NRP1+ ventricular progenitor cells for cardiac toxicity screening of test compounds are also provided.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.