Compositions and methods for site directed genomic modification

US11186843B2 · kind B2 · utility

Assignee

• Monsanto Technology LLC · US

Inventors

• Brent Brower-Toland · Chesterfield, US
• Andrei Y. Kouranov · Chesterfield, US
• Rosemarie Kuehn · Chesterfield, US
• Richard J. Lawrence · Fremont, US
• Ervin D. Nagy · Chesterfield, US
• Linda Rymarquis · High Ridge, US
• Veena Veena · Chesterfield, US

Key dates

• Filing date: Feb 27, 2015
• Grant date: Nov 30, 2021
• Expiry date: Aug 11, 2035

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12N15/8216
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.