Methods of engineering surface charge for bispecific antibody production
US11192951B2 · kind B2 · utility
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15Claims
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Key dates
| Filing date | Jun 4, 2018 |
| Grant date | Dec 7, 2021 |
| Priority date | — |
| Expiry date | Jun 4, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2317/94
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present disclosure relates to methods of modifying the isoelectric point of an antibody. The method includes providing an antibody comprising a first polypeptide comprising a heavy chain variable region and a second polypeptide comprising heavy chain variable region and substituting, in at least one of the first and second polypeptides of the antibody, one or more amino acid residues of the heavy chain variable region (VH) at positions 7, 9, 11, 14, 41, 70, 74, 82a, 84, and 113, according to the Kabat numbering system, wherein the substituting increases or decreases the isoelectric point of the antibody.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.