Methods to generate gastrointestinal epithelial tissue constructs

US11193110B2 · kind B2 · utility

Assignee

• The University of North Carolina at Chapel Hill · US

Inventors

• Nancy Allbritton · Irvine, US
• Yuli Wang · Lynnwood, US
• Christopher Sims · Irvine, US
• Scott Magness · Chapel Hill, US
• Scott Bultman · Lakewood, US

Key dates

• Filing date: Jan 29, 2016
• Grant date: Dec 7, 2021
• Expiry date: Jan 29, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2500/10
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

A method of making a live cell construct is carried out by: (a) providing a non-cellular support having a top surface and a bottom surface, (b) contacting live undifferentiated cells to the non-cellular support, and then (c) propagating a gastrointestinal epithelial cell monolayer on said top surface. In some embodiments, the live cells in the monolayer include: (i) undifferentiated cells (e.g., stem or progenitor cells); and (ii) optionally, but in some embodiments preferably, differentiated cells (e.g., enterocytes, Paneth cells, enteroendocrine cells, tuft cells, microcells, intra-epithelial lymphocytes, and/or goblet cells). Constructs formed by such methods and methods of using the same (e.g., in high through-put screening) are also described.