Methods for cleaving DNA and RNA molecules
US11193127B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jan 6, 2017 |
| Grant date | Dec 7, 2021 |
| Priority date | — |
| Expiry date | Mar 5, 2037 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/686
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein is a programmable RNA-activated DNA endonuclease activity associated with a Cmr complex. In one embodiment, the enzyme is a general double-stranded DNA endonuclease. Also provided is a programmable RNA endonuclease activity associated with a Cmr complex. In one embodiment, a Cmr2 protein present in a Cmr complex includes a mutation that reduces RNA-activated DNAse activity of the Cmr complex. In one embodiment, a Cmr4 protein present in a Cmr complex includes a mutation that reduces the RNase activity of the Cmr complex. Compositions including components of a Cmr complex and a CRISPR-RNA, and an optional activating RNA, are provided. Also provided are methods for using the compositions, and genetically engineered cells that include components of a Cmr complex and a CRISPR-RNA, and an optional activating RNA.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.