Nucleic acid-based linkers for detecting and measuring interactions
US11198900B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Nov 16, 2015 |
| Grant date | Dec 14, 2021 |
| Priority date | — |
| Expiry date | Mar 25, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2565/125
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention provides compositions comprising nucleic acid complexes for use in monitoring binding interactions and in measuring association and/or dissociation kinetics, detecting analytes including low concentration analytes, and screening library members. In some instances, the nucleic acid complexes are double-stranded nicked nucleic acids comprising a scaffold nucleic acid hybridized to one or more oligonucleotides. In some instances, a first, a second, a third, and optionally a fourth oligonucleotide are linked to moieties that are known to interact with each other or which are suspected of interacting with each other or of interacting with a common moiety such as an analyte. Changes in topology of the complex are used to determine the binding interactions of the various binding partners.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.