Analysis of chromatin using a nicking enzyme
US11198910B2 · kind B2 · utility
1Cited by
8References
11Claims
0Family size
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Key dates
| Filing date | Aug 31, 2017 |
| Grant date | Dec 14, 2021 |
| Priority date | — |
| Expiry date | Jul 22, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2600/154
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided herein, among other things, are various compositions and methods for analyzing chromatin. In some embodiments, the composition may comprise a mixture of a nicking enzyme, four dNTPs, at least one labeled dNTP and, optionally, a polymerase. In some embodiments, this method may comprise: obtaining a sample comprising chromatin, reacting the sample with the composition to selectively label the open chromatin in the sample, and analyzing the labeled sample.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.