Method for labeling specifically living bacteria comprising the use of modified non endogenous monosaccharide compounds
US11199542B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | May 3, 2016 |
| Grant date | Dec 14, 2021 |
| Priority date | — |
| Expiry date | May 8, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02A50/30
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The invention relates to a method for labeling specifically living bacteria, comprising the steps of: a) incubating said bacteria of said sample with at least one modified monosaccharide compound comprising a first reactive chemical group capable to chemically react with a second reactive group, so that a residue bearing said first reactive group is incorporated into the envelope of such bacteria, and b) contacting said modified monosaccharide residue incorporated within the envelope of the bacteria with a labeling molecule comprising a said second reactive group, for generating the chemical reaction of said first reactive group with said second reactive group, characterized in that said modified monosaccharide compound has the following formula (I), or a salt thereof: Wherein —A, B and C can be independently H, OH, NH2, OH and NH2 being substituted or not by protecting groups thereof and —D is an alkyl chain in C2 to C4, each carbon being substituted or not by OH or NH2, OH and NH2 being substituted or not by protecting groups thereof and —at least one of A, B, C or D groups is substituted by a said first reactive group.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.