Recombinant adeno-associated virus particle purification with multiple-step anion exchange chromatography
US11203740B2 · kind B2 · utility
1Cited by
3References
14Claims
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Key dates
| Filing date | Feb 9, 2016 |
| Grant date | Dec 21, 2021 |
| Priority date | — |
| Expiry date | Apr 9, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2750/14151
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
The invention describes a method for obtaining purified recombinant Adeno-Associated Virus particles (rAAV), comprising performing a depth filtration of a starting material previously obtained from cells producing rAAV particles comprising a cell lysate and/or a culture supernatant, followed by a first step of anion-exchange chromatography, a second step of anion-exchange chromatography and a final step of tangential flow filtration, whereby purified recombinant Adeno-Associated Virus particles (rAAV) are provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.