Microfluidic device and methods for digital assays in biological analyses
US11207686B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 21, 2018 |
| Grant date | Dec 28, 2021 |
| Priority date | — |
| Expiry date | Jul 22, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q1/6806
- WIPO fieldChemical engineering
- WIPO sectorChemistry
Abstract
An EWOD device and a related method of performing a digital biological assay are described that employs two volume measurements for enhanced assay determination. The method includes partitioning a sample reservoir and measuring the volume of each partition; initiating a biological assay wherein the biological assay includes measuring a partition property and a volume of each partition in real time as part of determining a concentration of the product substance in each partition based on the measured partition property and volume; and categorizing the partitions by a number of biological entities contained in each partition from which the number of biological entities may be calculated, which in turn may be used to calculate the total number of biological entities or concentration in the sample reservoir. The method further may include an enhanced partitioning process that minimizes variation in the volume of the partitions.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.