BRCA2-mediated purification of recombinase protein
US11208444B2 · kind B2 · utility
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14Claims
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Key dates
| Filing date | Feb 28, 2020 |
| Grant date | Dec 28, 2021 |
| Priority date | — |
| Expiry date | Feb 28, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC07K2319/24
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention is the products and methods associated with purifying overexpressed recombinant recombinases from a host cell line resulting in an un-tagged protein of interest without any additional, non-native amino acids. The invention employs at least one DNA vector that co-expresses a tagged fusion protein and the recombinase protein with the recombinase protein having an affinity for binding to the tagged fusion protein. Isolation methods of the recombinase protein include the targeting of the tagged fusion protein.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.