Cytochrome-fused glucose dehydrogenase and glucose measurement method
US11208466B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 30, 2016 |
| Grant date | Dec 28, 2021 |
| Priority date | — |
| Expiry date | Apr 1, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2333/904
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A cytochrome b-glucose dehydrogenase fusion protein having modified electron transfer properties, and a glucose measurement method and measuring kit using the cytochrome b-glucose dehydrogenase fusion protein are provided. Provided are a cytochrome b-glucose dehydrogenase fusion protein in which glucose dehydrogenase having homology with SEQ ID NO: 1 or SEQ ID NO: 4 and cytochrome b are linked together, as well as a glucose measurement method, a measurement reagent kit and a sensor using the cytochrome b-glucose dehydrogenase fusion protein. The cytochrome b-glucose dehydrogenase fusion protein of the present invention has modified electron transfer properties, and can be used for measuring glucose in the presence of a free-form mediator in reduced concentration or in the absence of a free-form mediator, and can be used, for example, in continuous glucose monitoring.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.