Patent · US Active

High throughput oil-emulsion synthesis of bowtie barcodes for paired mRNA capture and sequencing from individual cells

US11208681B2 · kind B2 · utility

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Key dates

Filing dateAug 14, 2017
Grant dateDec 28, 2021
Priority date—
Expiry dateAug 14, 2037

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q1/6869
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Methods for incorporation of unique bowtie-barcodes into a nucleic acid origami nanostructure (FIG. 1). In particular, provided herein are methods that facilitate pairing and analysis of nucleic acids from individual cells using, for example, high-throughput next-generation sequencing.

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