Integrated electrochemical detection and purification of nucleic acid biomarkers
US11209383B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 31, 2018 |
| Grant date | Dec 28, 2021 |
| Priority date | — |
| Expiry date | Dec 9, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N33/483
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
A biosensor platform apparatus and method are provided that can detect, purify and identify nucleic acid (DNA and RNA) biomarkers in complex biological fluids. The methods use a two-stage molecular based approach. The first stage screens for specific nucleic acid-based biomarkers in complex biological fluids by electrochemical detection of DNA:RNA hybridization and facilitates the removal of remaining complex media constituents. The first stage utilizes probes within a tunable nanoporous electrode. The second stage identifies the purified specific hybrids by single-molecule conductance measurements via break junction scanning. Identification can be assisted with a library of conductance measurements. The methods can provide strain level information that can be used for identifying anti-microbial resistance in detected pathogens. Collection of RNA targets allows for biomarker detection and identification without the need for amplification and can provide information about the viability of the sample organism.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.