Compositions and methods for detecting nicking enzyme and polymerase activity using a substrate molecule
US11220708B2 · kind B2 · utility
0Cited by
3References
30Claims
0Family size
Assignee
Inventor
Key dates
| Filing date | Feb 5, 2020 |
| Grant date | Jan 11, 2022 |
| Priority date | — |
| Expiry date | Feb 5, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y301/00
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides compositions and methods for assaying the activity of nicking enzyme and polymerase in a reaction involving the use of a nucleic acid substrate molecule that detects nicking enzyme and polymerase extension activities by the release of a detectable reporter (e.g., a fluorophore).
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.