Multi-copy strategy for high-titer and high-purity production of multi-subunit proteins such as antibodies in transformed microbes such as Pichia pastoris

US11225667B2 · kind B2 · utility

Assignee

• H. LUNDBECK A/S · DK

Inventors

• Danielle M. Mitchell · Seattle, US
• Leon F. Garcia-Martinez · Woodinville, US
• Patricia Dianne McNeill · Federal Way, US
• Ethan W. Ojala · Snohomish, US
• Mehmet Inan · Lincoln, US
• John Latham · Seattle, US

Key dates

• Filing date: Oct 29, 2018
• Grant date: Jan 18, 2022
• Expiry date: Jan 20, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C07K2319/50
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Methods for producing heterologous multi-subunit proteins in transformed cells are disclosed. In particular, the present disclosure provides improved methods of producing multi-subunit proteins, including antibodies and other multi-subunit proteins, which may or may not be secreted, with a higher yield and decreased production of undesired side-products. In exemplary embodiments, the transformed cells are a yeast, e.g., methylotrophic yeast such as Pichia pastoris.