Method for the detection, preparation and depletion of CD4+ t lymphocytes

US11226332B2 · kind B2 · utility

Assignee

• IMCYSE SA · BE

Inventors

• Jean-Marie Saint-Remy · Grez-Doiceau, BE
• Vincent Carlier · Énines, BE
• Luc Vander Elst · Obaix, BE

Key dates

• Filing date: Jul 10, 2019
• Grant date: Jan 18, 2022
• Expiry date: Dec 1, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2469/10
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates an in vitro method for detecting class II restricted CD4+ T cells in a sample. Herein a sample is contacted with an isolated complex of an MHC class II molecule and a peptide. This peptide comprises an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif. CD4+ T cells are detected by measuring the binding of the complex with cells in the sample, wherein the binding of the complex to a cell is indicative for the presence of CD4+ T cells in the sample. The present invention further relates to an isolated complex of an MHC Class II molecule and a peptide comprising an MHC class II restricted T cell epitope of an antigenic protein and immediately adjacent thereof, or separated by a linker of at most 7 amino acids a sequence with a [CST]-xx-C or C-xx-[CST] motif.