Methods for target DNA detection using non-functionalized carbohydrate-capped metallic nanoparticles
US11226335B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Mar 16, 2018 |
| Grant date | Jan 18, 2022 |
| Priority date | — |
| Expiry date | Apr 11, 2038 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Q2563/155
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The disclosure relates to a method for specific detection of a target analyte using probe DNA specific to the target analyte and non-functionalized, carbohydrate-capped metal nanoparticles such as non-functionalized, dextrin-capped gold nanoparticles. A sample mixture including a target DNA analyte and a probe DNA specific thereto is incubated to from a probe DNA-target DNA complex. The non-functionalized, carbohydrate-capped metal nanoparticles and an ionic species such as sodium chloride or other salt are added to the probe DNA-target DNA complex, and the mixture is incubated. Addition of the ionic species creates a detectable distinction, such as color of the resultant mixture, between stabilized metal nanoparticles when the probe DNA-target DNA complex is present and destabilized metal nanoparticles when the probe DNA-target DNA complex is absent. The method can be used for colorimetric detection of plant pathogens and associated diseases in agricultural production systems.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.