Patent · US Active

Coomassie Brilliant Blue Assay with improved sensitivity

US11226338B2 · kind B2 · utility

0Cited by

11References

11Claims

0Family size

Assignee

Siemens Healthcare Diagnostics Inc. · US

Inventors

Frank Vitzthum · Lahntal, DE
Herbert Schwarz · Niedereschach, DE
Anja Haude-Barten · Marburg, DE

Key dates

Filing date	Aug 10, 2017
Grant date	Jan 18, 2022
Priority date	—
Expiry date	Nov 24, 2038

Classification

Technology area (CPC G)Physics
CPC primaryG01N33/48
WIPO fieldMeasurement
WIPO sectorInstruments

Abstract

Methods for detection and quantitative measurement of proteins with a Coomassie Brilliant Blue Assay with improved sensitivity and maintaining high linearity over a broad measuring range are provided herein. In particular, a method of detecting a protein in a protein-containing sample is provided. The method includes providing a sample including a protein, a reagent including Coomassie Brilliant Blue and having a pH between 0.85 and 1.1, combining the sample and the reagent, and determining absorption at first and second wavelength to determine the amount of protein in the sample. The ratio of the absorption value at the first wavelength between about 580 to 620 nm to the absorption value at the second wavelength between about 520 to 370 nm is used in the spectral photometric determination of the amount of protein.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.