Large scale production of retinal pigment epithelial cells
US11230696B2 · kind B2 · utility
1Cited by
27References
27Claims
0Family size
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Key dates
| Filing date | Jul 28, 2016 |
| Grant date | Jan 25, 2022 |
| Priority date | — |
| Expiry date | Jul 28, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N2533/54
- WIPO fieldPharmaceuticals
- WIPO sectorChemistry
Abstract
A method of generating retinal pigment epithelial (RPE) cells is disclosed. The method comprises: (a) culturing human pluripotent stem cells in a human feeder cell-conditioned medium to obtain a cultured population of human pluripotent stem cells; (b) culturing said cultured population of human pluripotent stem cells in a medium comprising a differentiating agent to obtain differentiating cells; and (c) culturing said differentiating cells in a medium comprising one or more members of the TGFβ superfamily.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.