Method for determining the haemoglobin F content of an erythroid cell

US11231427B2 · kind B2 · utility

Assignees

• Université Paris—Est Créteil Val de Marne · FR
• Institut National de la Sante et de la Recherche Medicale (INSERM) · FR
• ETABLISSEMENT FRANCAIS DU SANG (EFS) · FR
• ASSISTANCE PUBLIQUE—HÔPITAUX DE PARIS (APHP) · FR

Inventors

• Marie Cambot · L'Haÿ-les-Roses, FR
• Gaetana Vandemeulebrouck · Paris, FR
• France Noizat Pirenne · Paris, FR
• Pablo BARTOLUCCI · L'Haÿ-les-Roses, FR
• Marie Georgine Rakotoson · Antananarivo, MG
• Frédéric Galacteros · Créteil, FR
• Nicolas Hebert · Ollainville, FR

Key dates

• Filing date: Nov 3, 2017
• Grant date: Jan 25, 2022
• Expiry date: Aug 7, 2038

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/52
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The invention concerns a method for determining, by flow cytometry, the hemoglobin F (HbF) content of each erythroid cell of a set of erythroid cells. This method applies in particular to determining the HbF content of each red blood cell of a set of red blood cells. The invention also concerns a method for determining the amount of red blood cells transfused into a patient and for monitoring the therapeutic efficacy of a treatment for sickle cell disease or β-thalassemia.