System and method for modeling and subtracting background signals from a melt curve

US11232851B2 · kind B2 · utility

Assignee

• Canon Information and Imaging Solutions, Inc. · US

Inventors

• Yang Yang · Beijing, CN
• Sophie Paquerault · Rockville, US
• Bradley Scott Denney · Irvine, US
• Lance Charlton · Rancho Santa Margarita, US
• Jeanette Paek · Cypress, US
• Attaullah Seikh · Irvine, US
• Vyshnnavi Parthasarathy · Irvine, US
• Ken Pearson · Buena Park, US
• Hung Huang · Irvine, US
• Tejinder Uppal · Orange, US

Key dates

• Filing date: Jun 23, 2017
• Grant date: Jan 25, 2022
• Expiry date: May 29, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2035/0091
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to methods for the analysis of nucleic acids present in biological samples, and more specifically to normalize a high resolution melt curve to assist in the identification of one or more properties of the nucleic acids. The present invention provides methods and systems that incorporate a background identification algorithm according to invention principles using raw melt curve data to identify reactions that are unrelated actual DNA melt reactions. Furthermore, a web-based application for analyzing experimental data is provided. The raw experimental data obtained from a variety of instruments is processed and analyzed on a server and presented to a user through a user interface (UI).