Method for constructing PD-1 gene modified humanized animal model and use thereof

US11234420B2 · kind B2 · utility

Assignee

• Biocytogen Pharmaceuticals (Beijing) Co., Ltd. · CN

Inventors

• Yuelei Shen · Beijing, CN
• Yang Bai · Beijing, CN
• Rui Huang · Beijing, CN
• Xiaofei Zhou · Beijing, CN
• Yuting Hu · Lo Wu, CN
• Yanan Guo · Beijing, CN
• Jichao Du · Beijing, CN

Key dates

• Filing date: May 31, 2019
• Grant date: Feb 1, 2022
• Expiry date: May 31, 2039

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2333/70532
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

Provided is a method for preparing a PD-1 gene-modified humanized animal model. The method utilizes the CRIPSR/Cas9 technique to replace partial fragments of a mouse PD-1 gene with fragments of a human PD-1 gene using homologous recombination by constructing a targeting vector, thereby preparing a gene-modified humanized mouse. This mouse can normally express a PD-1 protein containing the functional domain of the human PD-1 protein, and can be used as an animal model for mechanism research regarding PD-1, PD-L1 and other signals, for screening regulators, and for toxicological research. The method has an important and high application value in studies on functions of the PD-1 gene and in the development of new drugs.