Method for direct transformation of exogenous DNA into resting spores of Penicillium amagasakiense
US11236362B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 8, 2019 |
| Grant date | Feb 1, 2022 |
| Priority date | — |
| Expiry date | Feb 11, 2040 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12R2001/80
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention discloses a method for direct transformation of exogenous DNA into resting spores of Penicillium amagasakiense. The method includes three steps of culture of Penicillium amagasakiense and collection of spores, pretreatment of Penicillium amagasakiense spores, and electroporation of Penicillium amagasakiense spores by using HDEN method, to obtain Penicillium amagasakiense spores with introduction of plasmids to be transformed. In the present invention, non-germinated spores are used as a starting material for introduction of an exogenous molecule, and exogenous DNA is introduced into the resting spores of Penicillium amagasakiense by employing the HDEN electrotransformation technique, whereby the complex step of spore germination is omitted, and steps of protoplast preparation or Agrobacterium-mediated transformation in conventional methods etc. are omitted. Moreover, the transformation efficiency is high, and at least an effect of no less than 6000 positive transformants per transformation reaction system can be achieved.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.