Sensors for detection and quantification of microbiological protein secretion

US11236401B2 · kind B2 · utility

Assignee

• SenseUp GmbH · DE

Inventors

• Sarah-Kristin Jurischka · Düren, DE
• Georg Schaumann · Düsseldorf, DE
• Stephan Binder · Eschweiler, DE
• Britta Kleine · Gevelsberg, DE
• Roland Freudl · Düren, DE

Key dates

• Filing date: Mar 23, 2016
• Grant date: Feb 1, 2022
• Expiry date: Oct 29, 2036

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N21/6486
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

The present invention relates to a cell which is genetically modified with respect to its wild type and which comprises a gene sequence coding for a fluorescent protein, wherein the expression of the fluorescent protein depends on the amount of protein that is secreted across the cytoplasmic membrane into the extracytosolic space. The present invention also relates to a method for the identification of a cell having an increased secretion of protein across the cytoplasmic membrane into the extracytosolic space, a method for the identification of a culture medium composition that is optimized for the recombinant production of protein, a method for the identification of culture conditions that are optimized for the recombinant production of protein, a method for the identification of a compound that is characterized by an antibiotic activity due to its property to damage the membrane of a bacterial cell or to analyse the effect of such a compound on a population of genetically different bacterial cells or genetically identical cells in different physiological states or different growths phases, a method for the production of a cell which is genetically modified with respect to its wi…