Method and composition for detection of peptide cyclization using protein tags
US11237156B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Aug 15, 2017 |
| Grant date | Feb 1, 2022 |
| Priority date | — |
| Expiry date | Aug 15, 2037 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01J2219/00725
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
This invention relates to peptide microarrays, methods of generating peptide microarrays, and methods of identifying peptide binders using microarrays. More specifically, this invention relates to peptide microarrays, methods of generating peptide microarrays, and methods of identifying peptide binders using microarrays wherein the microarrays comprise cyclic peptides. The invention also relates to methods and compositions for detecting the formation of cyclized peptides from linear peptides on a microarray by contacting the microarray with a detectable protein. The cyclized peptides include tags that are activated upon cyclization, facilitating the detection of successful cyclization reactions. In additional aspects, the invention relates to developing fragmented peptide tags that, upon cyclization, bind to detectable proteins. Additionally, the invention relates to methods of generating linear and cyclic peptides subarrays on a microarray.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.