System and method for microscopic image processing and determining microglia activation state using processed images
US11238582B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Mar 12, 2020 |
| Grant date | Feb 1, 2022 |
| Priority date | — |
| Expiry date | May 20, 2040 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG06T2207/30024
- WIPO fieldComputer technology
- WIPO sectorElectrical engineering
Abstract
A method and system for preprocessing microscopic images. A method includes identifying a plurality of microglial cells shown in microglial cell channel images, wherein identifying the plurality of microglial cells further comprises quantizing the microglial cell channel microscopic images and filtering a plurality of objects in the quantized microglial cell channel microscopic images; identifying a soma and at least one projection for each of the plurality of microglial cells, wherein identifying the soma and the at least one projection for each microglial cell further includes iteratively removing pixels on the microglial cell as shown in one of the microglial cell channel images, wherein each soma and each projection has a respective size; and determining an activation state of each microglial cell based on the size of the soma and a total size of the at least one projection identified for the microglial cell.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.