Method for preparing porcine fibroblasts with both CD163 gene and CD13 gene being knocked-out

US11240997B2 · kind B2 · utility

Assignee

• Shandong Landsee Genetics Co., Ltd. · CN

Inventors

• Kui Li · Beijing, CN
• Yulian Mu · Beijing, CN
• Zhiguo Liu · Shanghai, CN
• Julang Li · Guelph, CA
• Kui Xu · Beijing, CN
• Yinghui Wei · Beijing, CN
• Changli Ge · Rizhao, CN
• Xuehui Cai · Jingwei, CN
• Long Wang · Rizhao, CN
• Qimei Chen · Nanhu, CN
• Yang Qiu · Blacksburg, US

Key dates

• Filing date: Apr 9, 2019
• Grant date: Feb 8, 2022
• Expiry date: Jul 18, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/124
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Provided is a double-gene knockout vector system, a method for preparing porcine fibroblasts with both CD163 gene and CD13 gene being knocked-out, prepared porcine fibroblasts, and a method for preparing a gene-edited pig with both CD163 gene and CD13 gene being knocked-out. The vector system of the present disclosure comprises a CD163 gene knockout vector and a CD13 gene knockout vector. The CD163 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, with a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 1-3. The CD13 gene knockout vector comprises a gene editing vector backbone and a DNA fragment ligated to the gene editing vector backbone, a nucleotide sequence of the DNA fragment being shown in any one of SEQ ID NOs: 4-6.