Engineered polymerases for improved sequencing
US11242512B2 · kind B2 · utility
Assignee
Inventor
Key dates
| Filing date | Sep 11, 2019 |
| Grant date | Feb 8, 2022 |
| Priority date | — |
| Expiry date | Sep 11, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12Y207/07049
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
Provided are nucleic acids encoding engineered polymerases comprising at least one modification in a motif A and/or at least one modification in a motif B of the polymerase and engineered polymerases encoded by the nucleic acids. Also provided are engineered DNA polymerases comprising a variant of SEQ ID NO:1, SEQ ID NO:2, or SEQ ID NO:3, the variant being at least 80% identical to SEQ ID NO:1, SEQ ID NO:2 or SEQ ID NO:3 and comprising an amino acid substitution at one or more positions selected from the group consisting of L408, Y409, P410, R484, A/L485, and I486. Methods, vectors, kits, and compositions comprising the nucleic acids and compositions, methods and kits comprising the engineered polymerases are also provided.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.