Method for constructing next-generation sequencing library for detection of lowfrequency mutation and kit thereof
US11248228B2 · kind B2 · utility
0Cited by
0References
3Claims
0Family size
Assignees
Inventors
Key dates
| Filing date | Mar 28, 2016 |
| Grant date | Feb 15, 2022 |
| Priority date | — |
| Expiry date | Jul 27, 2036 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/06
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention provides a method for constructing a next-generation sequencing library for detecting low-frequency mutations, and a kit thereof. The constructing method comprises steps of obtaining blunt-end DNA fragments, obtaining DNA fragments with A-tail at the 3′ end, obtaining adapter-added DNA fragments using a specific nucleotide sequence and obtaining amplification products using a specific nucleotide sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.