Method and system employing distinguishable polymerases for detecting ternary complexes and identifying cognate nucleotides

US11248254B2 · kind B2 · utility

Assignee

• OMNIOME, INC. · US

Inventors

• Corey M. Dambacher · Village of La Jolla, US
• Devon CAYER · Del Mar, US
• Richard LECOULTRE · San Diego, US
• Joseph Franklin Rokicki · San Diego, US
• Kerry Wilson · Elkhart, US
• Eugene Tu · San Diego, US
• Kandaswamy Vijayan · San Diego, US

Key dates

• Filing date: Dec 21, 2017
• Grant date: Feb 15, 2022
• Expiry date: Dec 29, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q1/6874
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Method of identifying a cognate nucleotide (i.e., the “next correct nucleotide”) for a primed template nucleic acid molecule. In some embodiments, an ordered or random array of primed target nucleic acids characterized by different cognate nucleotides can be evaluated using a single imaging step to identify different cognate nucleotides for a collection of different primed template nucleic acid molecules. An optional incorporation step can follow the identifying step. A polymerase different from the ones used in the binding and examination steps can be used to incorporate a nucleotide, such as a reversible terminator nucleotide, preliminary to identification of the next cognate nucleotide.