RNA fixation and detection in clarity-based hydrogel tissue
US11254974B2 · kind B2 · utility
1Cited by
28References
19Claims
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Key dates
| Filing date | Feb 9, 2017 |
| Grant date | Feb 22, 2022 |
| Priority date | — |
| Expiry date | Feb 9, 2037 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG02B21/0024
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Methods, kits, and systems for fixation of RNA permitting its detection in intact tissue, such as, large volume of mammalian tissue are disclosed. The methods, kits, and systems utilize carbodiimide-based chemistry to stably retain RNAs in tissue clarified using CLARITY. Also provided herein are methods, kits, and systems for detection of RNAs in clarified tissue.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.