Method for determining the concentration of a fluorescent and/or fluorescence-labeled analyte, and calibration method for preparing such determination

US11255784B2 · kind B2 · utility

Assignee

• Fraunhofer-Gesellschaft zur förderung der angewandten Forschung e.V. · DE

Inventors

• Benjamin GREINER · Rübhausen, DE
• Lorenz Sparrenberg · Bonn, DE
• Kristian Berwanger · Bonn, DE

Key dates

• Filing date: Oct 22, 2019
• Grant date: Feb 22, 2022
• Expiry date: Aug 11, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2201/127
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

A method for determining the concentration of a fluorescent and/or fluorescence-labeled analyte, and to a calibration method for preparing such determination, for use in the field of biological and environmental analysis in order to improve the accuracy of concentration determination, comprising the following steps: performing fluorescence measurements for calibration samples that have predetermined concentrations of a plurality of fluorescent and/or fluorescence-labeled reference analytes Rj that differ from each other by values m of a diffusion measure characterizing the diffusion of the reference analyte, in order to determine the values i of a concentration-dependent parameter I; establishing functions Fj(c)=i which describe the dependence of the parameter I on the concentration; determining the values of a slope parameter a for the respective reference analyte as a derivative of the respective function at c=0; determining the values mj of the diffusion measure for the reference analytes; establishing the dependence of the slope parameter a on the diffusion measure by a function E(m)=a; determining the value asample specific to the analyte using the value msample of the diffusi…