Methods for detecting 1,25-dihydroxyvitamin D and related antibodies

US11261257B2 · kind B2 · utility

Assignee

• DIASORIN S.p.A. · IT

Inventors

• Joshua Caine Soldo · Burnsville, US
• Gregory Olson · Plano, US
• Michael Lutterman · New Brighton, US
• John Wall · Limerick, IE
• Michael New · Bloomington, US
• Hector F. DeLuca · Deerfield, US
• Fabrizio Bonelli · Alessandria, IT

Key dates

• Filing date: Nov 12, 2019
• Grant date: Mar 1, 2022
• Expiry date: Mar 11, 2040

Classification

• Technology area (CPC G): Physics
• CPC primary: G01N2800/046
• WIPO field: Measurement
• WIPO sector: Instruments

Abstract

There is disclosed an assay method for selectively detecting 1,25-dihydroxy-vitamin D in a biological fluid sample. According to the method, the pH of the test sample is adjusted to 6-9 and a receptor protein comprising the Ligand Binding Domain of Vitamin D Receptor (VDR-LBD) is added to the test sample, thereby obtaining the formation of a VDR-LBD/1,25-dihydroxyvitamin D complex in which the VDR-LBD portion is conformationally changed with respect to unbound VDR-LBD. The VDR-LBD/1,25-dihydroxyvitamin D complex is then detected by means of a capture moiety which is capable of specifically binding to VDR-LBD bound to 1,25-dihydroxyvitamin D. Also disclosed are an assay kit and an antibody for carrying out the method. The assay is preferably a sandwich immunoassay.