Patent · US Active

Method for preparation of fungal mutant with high hydrolytic activity

US11261419B2 · kind B2 · utility

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6Claims
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Key dates

Filing dateDec 18, 2019
Grant dateMar 1, 2022
Priority date
Expiry dateMar 23, 2040

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12R2001/80
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

A method for preparing a hyper-cellulolytic catabolite derepressed mutants of ascomycetes fungus, especially variants of Penicillium funiculosum. Selection media used to isolate such variants include amorphous cellulose and a high concentration of glucose. Cellulase activities of mutant ID-10, in particular such as FPase and β-glucosidase were 1.5 times higher than Penicillium funiculosum MRJ-16 (parent). Furthermore, fungal mutant morphology was changed and no pH adjustment was required throughout the enzyme production process.

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