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Mevalonate diphosphate decarboxylase variants

US11261438B2 · kind B2 · utility

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Key dates

Filing dateJul 9, 2014
Grant dateMar 1, 2022
Priority date
Expiry dateDec 20, 2034

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Y401/01033
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

Described are mevalonate diphosphate decarboxylase variants having improved activity in converting 3-phosphonoxyisovalerate into isobutene. Such variants can be employed in processes for biologically producing isobutene from 3-hydroxyisovalerate or from 3-hydroxy-3-methylbutyrate into isobutene, for biologically producing isoprenol from mevalonate or from mevalonate-3-phosphate or for biologically producing 1,3-butadiene from 3-hydroxypent-4-enoate or from 3-phosphonoxypent-4-enoate.Also described is an enzyme which is characterized in that it is capable of converting 3-phosphonoxyisovalerate into isobutene with a kcat of more than 0.1 s−1.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.