Nannochloropsis spliced leader sequences and methods of use thereof
US11261452B2 · kind B2 · utility
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15Claims
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Assignee
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Key dates
| Filing date | Jan 23, 2018 |
| Grant date | Mar 1, 2022 |
| Priority date | — |
| Expiry date | Jan 31, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC12N15/67
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.