Method for recovering microbial cells
US11261476B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Oct 19, 2017 |
| Grant date | Mar 1, 2022 |
| Priority date | — |
| Expiry date | Jun 28, 2038 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2001/4088
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
The present invention provides a method of recovering viable microbial cells from a complex sample, said method comprising: a) providing a sample having a volume of at least 1 ml; b) contacting said sample with a buffer solution and one or more proteases, wherein said buffer solution has a pH of at least pH 6 and less than pH 11, wherein said buffer solution and said one more proteases do not comprise a detergent or a chaotrope, and wherein the buffer solution/protease/sample mixture is non-hypotonic; c) filtering the mixture obtained in step (b) through a filter suitable for retaining microbial cells; and d) recovering the microbial cells retained by the filter in step (c), wherein the recovered microbial cells are viable, and a microbial recovery device for the same.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.