Selective capture and release of rare mammalian cells using photodegradable hydrogels in a microfluidic platform
US11262361B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Jul 18, 2014 |
| Grant date | Mar 1, 2022 |
| Priority date | — |
| Expiry date | May 28, 2035 |
Classification
- Technology area (CPC B)Performing Operations; Transporting
- CPC primaryB01L2300/16
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein are photodegradable hydrogels and associated kits for selectively capturing and releasing cells. The hydrogels result from cross linking in the presence of a photoinitiator (1) a macromer having a polymeric backbone structure, a photo labile moiety, and a first linking moiety, and (2) a cell-binding moiety having a second linking moiety. These two components are cross-linked by a polymerization reaction of the linking moieties to form a photodegradable hydrogel incorporating the cell-binding moiety within the hydrogel. Also disclosed are methods of making the hydrogels, and methods of using the hydrogels for selectively capturing and releasing cells and for detecting cells in a fluid. Such methods can be used to detect the presence and quantity of certain rare cell types in a biological fluid.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.