Afucosylated protein, cell expressing said protein and associated methods

US11267899B2 · kind B2 · utility

Assignee

• Zumutor Biologics Inc. · US

Inventors

• Sohang Chatterjee · Kanchinakote, IN
• Kavitha Iyer Rodrigues · Bengaluru, IN
• Maloy Ghosh · Kanchinakote, IN
• Sunit Maity · Kanchinakote, IN
• Divya Unnikrishnan · Kanchinakote, IN
• Jahnabi Hazarika · Kanchinakote, IN
• Yogendra Manjunath Bangalore Muniraju · Kanchinakote, IN
• Sathyabalan Murugesan · Bengaluru, IN
• Pavithra Mukunda · Kundapura, IN
• Bhargav Prasad · Komangalam, IN
• Veeresha Kamanagowda · Kanchinakote, IN
• Sanghamitra Bhattacharjee · Kanchinakote, IN
• Pravin Kumar Dakshinamurthy · Komangalam, IN
• Vivek Halan · Viralimalai, IN
• Sankaranarayanan Srinivasan · Kanchinakote, IN
• Anuradha Hora · Sitapur, IN
• Bairavabalakumar Natarajan · Chennai, IN
• Karthika Nair · Survanagiri, IN
• Aswini Thanigaivel · Chennai, IN
• Amol Maliwalave · Survanagiri, IN
• Bharath Ravindra Shenoy · Kanchinakote, IN
• Sahana Bhima Rao · Savanuru, IN
• Subhra Prakash Chakrabarty · Survanagiri, IN
• Ashvini Kumar Dubey · Kanchinakote, IN
• Amir Khan · Aligarh, IN
• Ankurina Sharma · Savanuru, IN

Key dates

• Filing date: May 13, 2016
• Grant date: Mar 8, 2022
• Expiry date: May 19, 2038

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Y204/01068
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

The present disclosure relates to methods of obtaining cell with disrupted fucosylation and obtaining afucosylated protein. The present disclosure employs the Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) technology in a protein producing cell line to produce afucosylated protein. The resulting protein, specifically the resulting monoclonal antibody is completely afucosylated and reveals higher degree of antibody dependent cellular cytotoxicity.