Direct oligonucleotide synthesis on cells and biomolecules
US11268091B2 · kind B2 · utility
0Cited by
97References
7Claims
0Family size
Assignee
Inventors
Key dates
| Filing date | Dec 10, 2019 |
| Grant date | Mar 8, 2022 |
| Priority date | — |
| Expiry date | Dec 10, 2039 |
Classification
- Technology area (CPC C)Chemistry; Metallurgy
- CPC primaryC40B50/18
- WIPO fieldOrganic fine chemistry
- WIPO sectorChemistry
Abstract
The invention is directed to methods for synthesizing oligonucleotides directly on biomolecules or cells living or fixed. In some embodiments, template-free enzymatic synthesis is implemented under biological conditions with successive cycles of (i) enzymatic addition of a 3′-O-blocked nucleoside triphosphate and (ii) enzymatic deblocking of the incorporated nucleotide to regenerate a free 3′ hydroxyl. The invention has applications in single-cell cDNA library construction and analysis.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.