Methods of detecting gene fusions

US11268133B2 · kind B2 · utility

Assignee

• HTG Molecular Diagnostics, Inc. · US

Inventors

• Bruce Seligmann · Tucson, US
• BJ Kerns · Tucson, US
• John Luecke · Tucson, US
• Matt Rounseville · Tucson, US
• Ihab Botros · Tucson, US
• Mark Schwartz · Jeffersonville, US

Key dates

• Filing date: Apr 8, 2019
• Grant date: Mar 8, 2022
• Expiry date: Jun 20, 2039

Classification

• Technology area (CPC C): Chemistry; Metallurgy
• CPC primary: C12Q2600/156
• WIPO field: Biotechnology
• WIPO sector: Chemistry

Abstract

Disclosed herein are methods of detecting presence of a gene fusion in a sample from a subject. In some embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize a fusion probe that spans the point of fusion between two nucleic acids or genes. In other embodiments, the methods of detecting presence of a fusion gene in a sample from a subject utilize two or more probes that flank the point of fusion between two nucleic acids or genes. In additional embodiments, the methods can include determining the percentage of gene fusion in the sample relative to the first nucleic acid or the second nucleic acid.