Patent · US Active

Linked ligation

US11268137B2 · kind B2 · utility

1Cited by
43References
19Claims
0Family size

Assignee

Inventors

Key dates

Filing dateDec 7, 2017
Grant dateMar 8, 2022
Priority date—
Expiry dateApr 12, 2038

Classification

  • Technology area (CPC C)Chemistry; Metallurgy
  • CPC primaryC12Q2535/122
  • WIPO fieldBiotechnology
  • WIPO sectorChemistry

Abstract

The invention generally relates to capturing, amplifying, and sequencing nucleic acids. In certain embodiments, copies of the sense and antisense strands of a duplex template nucleic acid are captured using linked capture probes and multiple binding and extension steps to improve specificity over traditional single binding target capture techniques. Methods of seeding sequencing clusters with sense and antisense strands of a target nucleic acid are also disclosed including identifying the strands using sense-specific barcodes and confirming base calls using two sense-specific sequencing reads. Linked adapters may be used to increase adapter ligation selectively or efficiency and yield.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.