Patent · US Active

Protein sequencing methods and reagents

US11268963B2 · kind B2 · utility

3Cited by
2References
20Claims
0Family size

Inventor

Key dates

Filing dateOct 17, 2016
Grant dateMar 8, 2022
Priority date—
Expiry dateApr 23, 2038

Classification

  • Technology area (CPC Y)Emerging Cross-Sectional Technologies
  • CPC primaryY02P20/55
  • WIPO fieldMeasurement
  • WIPO sectorInstruments

Abstract

Described are optical methods and reagents for sequencing polypeptides. A probe that exhibits different spectral properties when conjugated to different N-terminal amino acids is conjugated to the N-terminal amino acid of a polypeptide. Sequentially detecting one or more spectral properties of the probe conjugated to the N-terminal amino acid and cleaving the N-terminal amino acid produces sequence information of the polypeptide. The use of super-resolution microscopy allows for the massively parallel sequencing of individual polypeptide molecules in situ such as within a cell. Also described are probes comprising hydroxymethyl rhodamine green, an isothiocyanate group and a protecting group.

Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.