Integrated microfluidic system for droplet generation, nucleic acid amplification, and detection
US11273444B2 · kind B2 · utility
Assignee
Inventors
Key dates
| Filing date | Nov 15, 2019 |
| Grant date | Mar 15, 2022 |
| Priority date | — |
| Expiry date | Nov 15, 2039 |
Classification
- Technology area (CPC G)Physics
- CPC primaryG01N2021/6471
- WIPO fieldMeasurement
- WIPO sectorInstruments
Abstract
Disclosed herein are microfluidic devices and systems for amplifying and detecting a target polynucleotide, comprising: one or more wells for receiving one or more substrates; a droplet generation channel in fluid communication with the one or more wells, wherein the microfluidic channel is adapted to generate droplets; and a chamber in fluid communication with the droplet generation channel, and adapted to collect droplets generated by the droplet generation channel, and further adapted to perform nucleic acid amplification in droplets, and further adapted to detect light signal from droplets. Also disclosed are methods of using the same.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.