Separation of enzymes from Trichoderma reesei by filter press and tangential filtration on a ceramic membrane
US11274122B2 · kind B2 · utility
Assignees
Inventors
Key dates
| Filing date | Jul 11, 2017 |
| Grant date | Mar 15, 2022 |
| Priority date | — |
| Expiry date | Jul 11, 2037 |
Classification
- Technology area (CPC Y)Emerging Cross-Sectional Technologies
- CPC primaryY02E50/10
- WIPO fieldBiotechnology
- WIPO sectorChemistry
Abstract
The invention relates to a method for separating, from a culture medium, an enzymatic cocktail and the fungus Trichoderma Reesei, the culture medium resulting from enzyme production by the fungus, method in which: said culture medium is subjected, in a period of no longer than 30 h from the halting of production, to a separation on a filter press lined with a fabric having a porosity of 3-20 μm, so as to obtain a filtrate having a corrected optical density OD at 600 nm of less than 2.5; and the liquid phase obtained is subjected to tangenital micro-filtration on a ceramic membrane having a cut-off limit of 0.5 to 1.4 μm, so that the corrected OD at 600 nm does not exceed 0.1.
Source: USPTO / EPO open patent data. Objective bibliographic and citation counts.